Journal of Pharmacy Research, Vol 5, No 8 (2012)

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Sep 01
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Simultaneous estimation of emtricitabine, tenofovir disoproxil fumarate, and rilpivirine in bulk form by RP-HPLC method

D.Pranitha1, Vanitha C1*, Prince Francis1, M. Alagar Raja1, P. Vishnu vardan1, Surendar.M1, David Banji1

Abstract


A new simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the determination of Emtricitabine, Tenofovir disoproxil fumarate, Rilpivirine. The Method employs Thermo Hypersil ODS C-18 column (150×4.6mm, 5µ) and flow rate of 1 ml/min with a load of 20µl. Acetonitrile and Phosphate buffer PH 3was used as mobile phase in the composition of 60:40. The Detection was carried out at 260 nm. Linearity ranges for Emtricitabine, Tenofovir disoproxil fumarate, Rilpivirine were 8-48µg/ml, 12-72µg/ml, 1-6µg/ml respectively. Retention Time of Emtricitabine, Tenofovir disoproxil fumarate, Rilpivirine were found to be 2.408 min, 3.664 min, 12.062 min respectively. Percent Recovery study values of Emtricitabine, Tenofovir disoproxil fumarate, Rilpivirine were found to be within 98-102%.This newly developed method was successfully utilized for the Quantitative estimation of Emtricitabine, Tenofovir disoproxil fumarate, Rilpivirine in bulk form. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines.

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